microRNAs (miRNAs) are endogenous non-coding RNA whose length is about
19-25nt, they are widely involved in the regulation of gene transcription.
However, miRNA qRT-PCR and miRNA microarray pay more attention to the miRNA
expression and quantitative, they are limited to the detection of known miRNA
expression profiles but have no way to detect novel miRNA molecules.
Using the next-generation sequencing technology platform can directly
high-throughput sequence the specified size miRNA molecules in the sample, and
can study the miRNA expression profiling without any miRNA sequence information,
discover and identify new miRNA molecules, so as to provide more comprehensive
miRNA expression study method and accelerate the discovery of new miRNA.
The next-generation sequencing technology is a powerful tool for miRNA
RiboBio miRNA team will provide miRNA high-throughput sequencing services of
cells to clients based on professional experience.
Digital signal: miRNA high-throughput sequencing platforms can study the
miRNA molecules directly from the single nucleotide level without hybridization
signals obtained by traditional chip, this can ultimately avoid the problem of
background signal noise, and is useful for distinct miRNA families.
High sensitivity: The ability to detect low-abundance miRNAs, and each
sequencing channel can detect tens of millions of miRNA data with high
High-quality data: miRNA sequencing has a large amount of sequencing flux and
can provide sufficient sequencing depth and data coverage for each miRNA
molecules, the results are reliable and accurate.